top-down proteomics
Researchers including Northwestern's Neil Kelleher and UVA's Gloria Sheynkman are exploring whether the platform could complement their mass spec analyses.
While the method is still in its early stages, it points the way toward a very high-throughput workflow for top-down single-cell proteomics.
The aim is to improve the accuracy of the protein measurements commonly reported as the output of proteomic experiments and uncover obscured biological signals.
The study used analysis of intact proteins to identify mutation-linked post-translational modifications involved in KRAS activation and membrane attachment.
Writing in MCP, the researchers provided a survey of techniques for biologists interested in using multiplexed quantitative proteomics in their research.