single-molecule sequencing
Recent advancements in long-read sequencing are enabling researchers to study full-length mRNA isoforms at the single-cell level.
The most recent software, Paraphase, produces "mini haplotypes" rather than counting copy number variations to identify variants linked to rare diseases.
SMS-seq combines structural probing with direct RNA nanopore sequencing, offering researchers a new way to study RNA structure at the single-molecule level.
The sequencing-by-synthesis platform, using technology originally developed by Helicos, was approved by China's NMPA for noninvasive prenatal testing earlier this year.
The technology builds on the zero-mode waveguide nanotechnology underlying Pacific Biosciences' sequencing method and could be used for RNA-seq.