The researchers say that methods that include gene expression as well as other genomic data in one assay could help unravel cell function and gene regulation.
The SCN3A gene, which encodes a voltage-gate sodium channel in the brain, also controls oral motor development, including swallowing, tongue movement, and speech.
Single-cell RNA sequencing from mouse trachea and human bronchial epithelial cells unearthed new cell types, including ionocytes enriched for CFTR expression.
The technique features sequencing-by-ligation and error-correction strategies, and was used to simultaneously map more than 1,000 genes in mouse brain tissue.
