Close Menu

single-cell sequencing

By Monica Heger

This article was originally published March 19.

By Monica Heger
Despite the technical challenges of single-cell sequencing, researchers have begun using the technique to better study single bacterial species from metagenomic samples.

The competition, part of a $7 million "Grand Challenges" program that Life Tech launched last year, will award a $1 million prize to the first SOLiD user who succeeds in sequencing the genome and transcriptome of a single cancer cell.

There are two main problems when it comes to sequencing single cells: Whole-genome amplification protocols do not amplify the genome in an unbiased manner, and PCR steps in library preparation introduce additional biases and errors.

By sequencing individual cells from primary and metastatic breast cancer samples and using this data to glean copy number patterns in the cells, researchers have gained insights into how these cancers evolved and spread.

Researchers from Cold Spring Harbor Laboratory reported at last week's Biology of Genomes meeting that they sequenced 100 cells from a basal-like breast cancer tumor to reveal five major subpopulations of cells, three of which were cancerous.

Pages

Mainichi reports that 43 percent of Japanese individuals said they did not want to eat agricultural products that had been modified using gene-editing tools.

Two US Department of Agriculture research departments are moving to the Kansas City area, according to the Washington Post.

Slate's Jane Hu compares some at-home genetic tests to astrology.

In PLOS this week: analysis of polygenic risk scores for skin cancer, chronic pain GWAS, and more.