SILAC | GenomeWeb

SILAC

Writing in MCP, the researchers provided a survey of techniques for biologists interested in using multiplexed quantitative proteomics in their research.

A team of Roche researchers has developed a five-plex SILAC method for quantifying the dynamics of tyrosine phosphorylation.

This story has been updated to correct an earlier version that identified Steven Gygi as an MIT researcher. He is a researcher at Harvard University.

A team led by researchers at the European Molecular Biology Laboratory has developed a new method for quantitative measurement of the secreted proteome, or secretome.

The screens, done independently by one Harvard-led and one MIT-led research team, both identified the protein Grb10 as an mTOR substrate and a likely tumor suppressor involved in regulating PI3K activity.

In particular, the study focused on signaling networks tied to CTL chromatin regulation with the aim of better understanding the systems of gene regulation characteristic of these cells, said one of the researchers.

The study showed that EGF signaling impacts ubiquitination across a wide range of protein networks and is one of the first to demonstrate cross-talk between ubiquitin-regulated and phospho-regulated signaling pathways, said one of the scientists.

Their study, Mann said, suggests that protein expression is more similar across cell types than previously thought — a finding with implications for drug development as well as basic cell biology research.

The collaboration, which aims to characterize the tryptic peptides of the 5,000 proteins in order to generate mass spectroscopy assays for them, brings together ISB's mass spectroscopy expertise and OriGene's extensive human protein collection.

In a study published in Nature Methods, Matthias Mann and his colleagues at the Max Planck Institute for Biochemistry describe a method they have developed to expand the use of SILAC for tissue samples and eventually to researchers who may not be specialists in mass spectrometry.

Biomedical research projects are generating a ton of data that still needs to be analyzed, NPR reports.

Theranos is retiring some of its board members, including Henry Kissinger and George Shultz, Business Insider reports.

The heads of 29 scientific societies and some 2,300 researchers call on President-elect Donald Trump to rely on and support science in two separate letters.

In Science this week: genetically modified flu virus could be key to new live vaccines, and more.