methylation-specific PCR

NEW YORK (GenomeWeb) — Iowa City-based Behavioral Diagnostics recently won a one-year, $200,000 Small Business Innovation Research grant from the National Institute on Drug Abuse to fund development of its quantitative measure of cigarette smoking and cessation.

NEW YORK (GenomeWeb) — Biochemists at the University of Konstanz in Germany have created a mutant polymerase with high activity and high mismatch extension selectivity. The new enzyme is a variant of the large Klenow fragment of Thermus aquaticus DNA polymerase, or KlenTaq.

Researchers affiliated with the laboratory of Mike Makrigiorgos at Dana Farber Cancer Institute and Harvard Medical School have demonstrated how their COLD-PCR method can be applied to epigenetics, allowing the enrichment and sequencing of rare unmethylated DNA regions in a background of highly m

A study published this week has shown methylation status in a panel of genes may be useful to non-invasively monitor breast cancer recurrence and treatment response.

With numerous kits on the market for PCR of methylated DNA, researchers may wonder which is the best for bisulfite conversion of various types of real-world samples.