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library prep

Earlier this month, three early adopters described their use of the method in Genome Biology, including a comparison with standard library prep protocols, sub-nanogram library construction, exome capture from nanograms of DNA, PCR-free libraries, colony PCR libraries, and 96-plex barcoding.

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Beckman Coulters' SPRIworks Fragment Library System, Complete Genomics' CNV and SV analysis, Ambry Genetics' sequencing services on HiSeq 2000, EdgeBio a CSP for Agilent's SureSelect Target Enrichment System

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IntergenX's Apollo 324 system

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IntegenX launches Apollo 324 system for automated DNA library prep

Both companies' platforms are alternatives to traditional gel electrophoresis methods used in library preparation for next-gen sequencing.

At a recent conference, two members of the Broad's process development team shared some of the changes that have made sample prep faster, cheaper, and more high-throughput.

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SureSelect Human All Exon 50Mb Target Enrichment Kit, Sage Science Pippin Prep DNA size selection system

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Edison Liu, Michael Quail, Vincent Magrini, Patrick Cahill, Christopher Detter, Jan Fang Cheng, John Gill, Karen Beeson, Ray Stata, Raynard Kington

Over the next year or so, Polonator developers plan additional improvements, including reagent kits, increased read lengths, and a simpler library preparation protocol. By 2010, they expect the output per run to increase tenfold, to 100 gigabases.

Although the developers note that the protocol particularly benefits the sequencing of AT-rich genomes, they say that it is also quicker than the standard method, which requires PCR amplification, and "should be used routinely to prepare libraries for Genome Analyzer sequencing."

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