Earlier this month, three early adopters described their use of the method in Genome Biology, including a comparison with standard library prep protocols, sub-nanogram library construction, exome capture from nanograms of DNA, PCR-free libraries, colony PCR libraries, and 96-plex barcoding.
Beckman Coulters' SPRIworks Fragment Library System, Complete Genomics' CNV and SV analysis, Ambry Genetics' sequencing services on HiSeq 2000, EdgeBio a CSP for Agilent's SureSelect Target Enrichment System
Over the next year or so, Polonator developers plan additional improvements, including reagent kits, increased read lengths, and a simpler library preparation protocol. By 2010, they expect the output per run to increase tenfold, to 100 gigabases.
Although the developers note that the protocol particularly benefits the sequencing of AT-rich genomes, they say that it is also quicker than the standard method, which requires PCR amplification, and "should be used routinely to prepare libraries for Genome Analyzer sequencing."