iTRAQ | GenomeWeb


New Products


Applied Biomics this week added Isobaric Tags for Relative and Absolute Quantitation, or iTRAQ, services to its line of proteomics offerings.

The screens, done independently by one Harvard-led and one MIT-led research team, both identified the protein Grb10 as an mTOR substrate and a likely tumor suppressor involved in regulating PI3K activity.

The analytical strategy, which combines CID and HCD, provides sensitive and accurate protein identification, as well as isobaric-labeling-based quantitation, specifically iTRAQ labeling, on ion trap mass specs and LTQ Orbitraps, its developers said.

In an evaluation on the MCF-7 breast cancer cell line cultured in 17-beta estradiol and tamoxifen, the method detected 530 proteins and quantified 230 of them. The researchers selected 16 differentially expressed proteins "demonstrating the potential of iTRAQ/PQD-MS for biomarker discovery applications."

Harvard's George Church discusses HGP-write with the Journal of the American Medical Association.

In Nature this week: genetic history of HIV in the US, and more.

There are a few projects aimed at addressing the lack of diversity in genomic research, Technology Review reports.

A national assessment shows that US students lag in the sciences, but suggests that achievement gaps are narrowing.