iTRAQ

New Products

Premium

Applied Biomics this week added Isobaric Tags for Relative and Absolute Quantitation, or iTRAQ, services to its line of proteomics offerings.

The screens, done independently by one Harvard-led and one MIT-led research team, both identified the protein Grb10 as an mTOR substrate and a likely tumor suppressor involved in regulating PI3K activity.

The analytical strategy, which combines CID and HCD, provides sensitive and accurate protein identification, as well as isobaric-labeling-based quantitation, specifically iTRAQ labeling, on ion trap mass specs and LTQ Orbitraps, its developers said.

In an evaluation on the MCF-7 breast cancer cell line cultured in 17-beta estradiol and tamoxifen, the method detected 530 proteins and quantified 230 of them. The researchers selected 16 differentially expressed proteins "demonstrating the potential of iTRAQ/PQD-MS for biomarker discovery applications."

This year's Breakthrough Prize winners include a pair that developed a therapy for spinal muscular atrophy.

The New York Times reports on how white supremacists misconstrue genetic research, concerning many geneticists.

Researchers find that people's genetics influence their success at university, but that it is not the only factor.

In Nature this week: approach to identify genetic variants that affect trait variability, application of read clouds to microbiome samples, and more.