iTRAQ
New Products: Sep 30, 2011
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Applied Biomics this week added Isobaric Tags for Relative and Absolute Quantitation, or iTRAQ, services to its line of proteomics offerings.
The screens, done independently by one Harvard-led and one MIT-led research team, both identified the protein Grb10 as an mTOR substrate and a likely tumor suppressor involved in regulating PI3K activity.
The analytical strategy, which combines CID and HCD, provides sensitive and accurate protein identification, as well as isobaric-labeling-based quantitation, specifically iTRAQ labeling, on ion trap mass specs and LTQ Orbitraps, its developers said.
In an evaluation on the MCF-7 breast cancer cell line cultured in 17-beta estradiol and tamoxifen, the method detected 530 proteins and quantified 230 of them. The researchers selected 16 differentially expressed proteins "demonstrating the potential of iTRAQ/PQD-MS for biomarker discovery applications."