iTRAQ

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Applied Biomics this week added Isobaric Tags for Relative and Absolute Quantitation, or iTRAQ, services to its line of proteomics offerings.

The screens, done independently by one Harvard-led and one MIT-led research team, both identified the protein Grb10 as an mTOR substrate and a likely tumor suppressor involved in regulating PI3K activity.

The analytical strategy, which combines CID and HCD, provides sensitive and accurate protein identification, as well as isobaric-labeling-based quantitation, specifically iTRAQ labeling, on ion trap mass specs and LTQ Orbitraps, its developers said.

In an evaluation on the MCF-7 breast cancer cell line cultured in 17-beta estradiol and tamoxifen, the method detected 530 proteins and quantified 230 of them. The researchers selected 16 differentially expressed proteins "demonstrating the potential of iTRAQ/PQD-MS for biomarker discovery applications."

Sometimes genetic tests give inconclusive results and provide little reassurance to patients, the Associated Press reports.

Vox wonders whether gene-editing crops will be viewed similarly as genetically modified organisms of if people will give them a try.

In Science this week: research regulation and reporting requirement reform, and more.

With H3Africa, Charles Rotimi has been working to bolster the representation of African participants and African researchers in genomics, Newsweek reports.