iTRAQ

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Applied Biomics this week added Isobaric Tags for Relative and Absolute Quantitation, or iTRAQ, services to its line of proteomics offerings.

The screens, done independently by one Harvard-led and one MIT-led research team, both identified the protein Grb10 as an mTOR substrate and a likely tumor suppressor involved in regulating PI3K activity.

The analytical strategy, which combines CID and HCD, provides sensitive and accurate protein identification, as well as isobaric-labeling-based quantitation, specifically iTRAQ labeling, on ion trap mass specs and LTQ Orbitraps, its developers said.

In an evaluation on the MCF-7 breast cancer cell line cultured in 17-beta estradiol and tamoxifen, the method detected 530 proteins and quantified 230 of them. The researchers selected 16 differentially expressed proteins "demonstrating the potential of iTRAQ/PQD-MS for biomarker discovery applications."

A former Synthetic Genomics attorney alleges that the firm discriminated against her and other female employees, according to the San Diego Union-Tribune.

Due to privacy and lab certification questions, the planned giveaway of Orig3n testing kits at a Baltimore Ravens game was suspended.

Alnylam reports positive results from its phase 3 clinical trial of an RNAi-based drug, according to Stat News.

In Cell this week: adult mesenchymal cell populations in mouse lung, genetic diversity in HPV16 and cancer risk protection, and more.