isobaric tagging
However, a shift within the field towards experiments comprising larger numbers of samples and conditions may give DIA an advantage in the future.
The reagents are intended for use in a recently developed workflow designed to address the precursor interference issue that has troubled the labeling method.
At the National Center for Quantitative Biology of Complex Systems, scientists hope mass spec will drive new biological questions and vice versa.
Writing in MCP, the researchers provided a survey of techniques for biologists interested in using multiplexed quantitative proteomics in their research.
The reagents, which use combinations of reporter ions, could allow for multiplexes of up to 28 samples, more than double the capacity of existing tags.