These hybrid capture-based methods target gene transcripts of interest, which could shrink sequencing costs for some experiments by as much as 90 percent.
The team also found that the proportion of infected people didn't change substantially during the screening period, consistent with beneficial containment.
The screen, which multiplexes Cas9 and Cas12a, could be used for functional analysis of large genomic regions in mammalian cells, the researchers said.
Two recent CRISPR-Cas9 viability screens in cancer cell lines were concordant across multiple metrics despite significant differences in experimental protocols.
In Science this week: approach to measure microRNA targeting efficiency, strategy to conduct high-throughput chemical screens at single-cell resolution, and more.
The new method, called sci-Plex, combines nuclear hashing and improved single-cell combinatorial indexing RNA-seq to profile the transcriptional response of single cells.
