droplet-based PCR | GenomeWeb

droplet-based PCR

A proof-of-principle study has shown a new qPCR device can detect bacterial DNA in under four minutes using droplet size as a readout of amplification.

The company has established a streamlined workflow that customizes and automates key steps of the sample prep process.

The simple, semi-open format uses a microfluidics robot to generate an array of picoliter droplets on a reusable chip. 

The test, which is being developed with a National Science Foundation grant, is based on a novel PCR technology that doesn't require traditional instrumentation.

Researchers from University College London, have shown that a targeted bisulfite sequencing method using RainDance Technologies' microdroplet PCR system can accurately measure DNA methylation levels at sample sizes as low as 100 nanograms.

Researchers from Northwestern University and the University of Chicago have demonstrated in a new study that droplet-based digital PCR offers the ability to detect BCR-ABL1 fusion transcripts with a lower limit of detection and quantification than currently used quantitative PCR

In a study published this month, Bio-Rad researchers have demonstrated the capability of the company's new droplet digital PCR system using DNA-binding dyes with comparable results to TaqMan and that it's possible to use this new dye-based approach for the quantification of multi

University of Arizona scientists have developed a "wire-guided" droplet-based PCR method that they claim can amplify and detect gene targets in real time in about three minutes.

By Julia Karow GnuBio plans to release a beta version of its desktop microfluidic sequencer in the second quarter and to launch the system commercially by the end of the year, the company said earlier this month.

An opinion piece in the New York Times urges lawmakers to keep genetic protections in place.

Research funding in Canada is to remain mostly the same, ScienceInsider reports.

In Science this week: random DNA replication errors play role in cancer, and more.

The Bill and Melinda Gates Foundation embarks on an open-access publishing path.