bisulfite sequencing
New Enzymatic Approach Enables Transcriptome-Wide N6-Methyladenosine Profiling, Quantification
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The method, dubbed eTAM-seq, offers scientists a new way to study m6A modifications in mRNA at a high resolution while lowering the sample input requirement.
The approach aims to discern environmental DNA contamination by chemically tagging sample-intrinsic cfDNA prior to DNA extraction using bisulfite salt treatment.
Pan-Cancer Analyses Reveal Copy Number Signatures, Chromosomal Instability Patterns
Investigators identified up to 21 copy number signatures in cross-cancer analyses, including potential disease response markers and signatures linked to chromosomal instability features.
With bisulfite whole-genome sequencing of cfDNA from blood, researchers were able to monitor graft-versus-host disease, infection, graft failure, and disease relapse.
Thyroid Size, Function in Children Linked to Nutrition-Related DNA Methylation Mark
A trial in Gambia suggests PAX8 gene methylation in 2-year-olds corresponds to thyroid volume, hormone production, and physical features later in childhood.