As Users Demand Paired-End Sequencing, 454, Illumina, and ABI Work On New Kits | GenomeWeb
For next-generation sequencing, paired reads — two stretches of sequenced DNA with an unsequenced insert of known size in between them — seem to be indispensable.
While paired reads have been crucial in Sanger sequencing for applications like de novo assemblies, “for short reads they are even more important as they add significant specificity,” said Chad Nusbaum, co-director of the genome sequencing and analysis program at the Broad Institute, in an e-mail message to In Sequence.

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