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TransPlex Whole Transcriptome Amplification WTA2, Bioconductor 2.3, TC-3000X

Sigma-Aldrich has released TransPlex Whole Transcriptome Amplification technology, WTA2, for amplifying total RNA from a variety of tissue sources such as animals, plants, and microorganisms. WTA2, which is optimized for formalin-fixed and paraffin-embedded tissue samples, creates a cDNA library from total RNA and amplifies the cDNA in a single PCR reaction. TransPlex reagents and protocols, optimized to ensure clonal and stochastic amplification of all expressed genes and exons, allow RNA samples to be amplified 1,000 to 10,000-fold in less than four hours, according to the company. The technology is designed for use with RNA that is partially degraded, as in formalin-fixed paraffin-embedded tissue derived from tumor samples.

Bioconductor 2.3 is available here. The new release includes 36 new software packages as well as changes to existing packages. Seven new packages — BSgenome, Biostrings, ShortRead, IRanges, HilbertVis, HilbertVisGUI, and rtracklayer — are suited to analyze second-generation DNA sequence data. In total, Bioconductor 2.3 comprises 294 software packages and is compatible with the recently released R 2.8.0.

Bibby Scientific has launched TC-3000X, a new thermal cycler that is part of its Techne brand. The instrument has two interchangeable block options, a 48-well block for 0.2 ml tubes or a 30-well block for 0.5 ml tubes. The defined-pressure, heated lid is height-adjustable.

The Scan

Renewed Gain-of-Function Worries

The New York Times writes that the pandemic is renewing concerns about gain-of-function research.

Who's Getting the Patents?

A trio of researchers has analyzed gender trends in biomedical patents issued between 1976 and 2010 in the US, New Scientist reports.

Other Uses

CBS Sunday Morning looks at how mRNA vaccine technology could be applied beyond SARS-CoV-2.

PLOS Papers Present Analysis of Cervicovaginal Microbiome, Glycosylation in Model Archaea, More

In PLOS this week: functional potential of the cervicovaginal microbiome, glycosylation patterns in model archaea, and more.