Users of second-generation sequencing platforms from Illumina, 454/Roche, and Life Technologies/Applied Biosystems witnessed improvements in yield, read length, and data quality in 2008, though many struggled with technical problems relating to their instrument or reagents, according to a survey conducted by In Sequence at the end of 2008.

Also, the $10,000 human genome remained elusive in 2008, as the total cost for users to generate a gigabase of high-quality data ranged between $1,600 and $146,500 at the end of the year, depending on the platform.

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