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Takara, Formulatrix Develop Miniaturized Single-Cell Sequencing Workflows

NEW YORK (GenomeWeb) – Takara Bio and Formulatrix today announced that they have developed standard operating procedures (SOPs) for miniaturizing reaction volumes in the reverse transcription portions of single-cell sequencing workflows.

According to the companies, the SOPs detail how to prepare and combine reagents to build Takara's SMART-Seq v4 cDNA libraries using Formulatrix's MANTIS liquid handling platform.

In a statement, Takara Bio USA President Carol Lou said the workflow allows researchers to use 25 percent of the primers and enzymes required by conventional methods, while also analyzing three to four times as many cells.

The Scan

Cell Studies on Tumor Evolution in Mouse Model of Lung Cancer, Stereo-seq, Bacteriophage Responses

In Cell this week: tumor evolution tracked in mouse model of lung cancer, organogenesis mapped using Stereo-seq, and more.

Taking Stock of the Stockpile

The US and European countries are evaluating their smallpox vaccine stockpiles as the number of monkeypox cases increases, the Washington Post reports.

Vitamin D From Tomatoes

According to Reuters, researchers in the UK have gene-edited tomatoes so their fruit contains vitamin D.

Cause Not Yet Spotted

NPR reports that a new study was unable to find a cause for persistent long COVID symptoms.