Stanford Researchers Identify the Essential Genome of Caulobacter Crescentus | GenomeWeb

To suss out which stretches of the Caulobacter crescentus genome are necessary for its function, Lucy Shapiro and her group at Stanford University oversaturated it with transposons. If the transposon lands in, and disrupts, a region of the Caulobacter crescentus genome that is essential for bacterial function, then that clone cannot be grown on rich media, while bacteria with non-essential disruptions can. "We are looking for the dog that doesn't bark," Shapiro says.

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