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SOLiD Whole Transcriptome Expression Kit

Applied Biosystems said that it plans to launch a SOLiD Whole Transcriptome Expression kit as part of an early-access program in November. The new kit, which is based on Ambion technology, allows for up to 10 RNA libraries to be sequenced simultaneously. The technology conserves strandedness of cDNA. Using the kit, researchers first isolate and purify total RNA or mRNA from cell or tissue samples. Ribosomal RNA is then removed from these samples, followed by a RNA fragmentation step. Researchers then ligate adapters the RNA and convert it into cDNA, which is sequenced on the SOLiD system. ABI said it will also make data analysis tools available.

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.