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SOLiD Whole Transcriptome Expression Kit

Sep 02, 2008
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Applied Biosystems said that it plans to launch a SOLiD Whole Transcriptome Expression kit as part of an early-access program in November. The new kit, which is based on Ambion technology, allows for up to 10 RNA libraries to be sequenced simultaneously. The technology conserves strandedness of cDNA. Using the kit, researchers first isolate and purify total RNA or mRNA from cell or tissue samples. Ribosomal RNA is then removed from these samples, followed by a RNA fragmentation step. Researchers then ligate adapters the RNA and convert it into cDNA, which is sequenced on the SOLiD system. ABI said it will also make data analysis tools available.

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Clinical Sequencing
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