Skip to main content
Premium Trial:

Request an Annual Quote

Sequencing-Related US Patents Granted Nov. 28 – Jan. 1

Premium
US Patent 7,315,019. Arrays of optical confinements and uses thereof.
Inventors: Stephen Turner, Jonas Korlach. 
Assignee: Pacific Biosciences.
 
The invention relates to optical confinements, and methods of preparing and using them for analyzing molecules and for monitoring chemical reactions. The apparatus and methods embodied in the invention are particularly useful for high-throughput and low-cost single-molecular analysis, according to the patent.
 

 
US Patent 7,313,308. Optical analysis of molecules.
Inventors: Stephen Turner, Jonas Korlach.
Assignee: Pacific Biosciences.
 
The invention relates to optical confinements, and methods of preparing and using them for analyzing molecules and monitoring chemical reactions. The apparatus and methods embodied in the invention are particularly useful for high-throughout and low-cost single-molecular analysis, according to the patent.
 

 
US Patent 7,312,054. Methods and reaction mixture reagent for increasing the specificity and fidelity of polymerase reactions.
Inventor: Martin Mautner.
Assignee: Biodynamics.
 
Describes a method for reducing the efficiency of primer extension by polymerase enzymes when the 3' end of a primer or growing nucleic acid chain does not hybridize perfectly with the target, for increasing the selectivity of single nucleotide mutation or gene analyses, for suppressing false positive results and for enhancing the fidelity of the amplification of nucleic acid fragments by avoiding the incorporation of mispairs. The method involves obtaining a nucleic acid sample, hybridizing it to a primer, subjecting it to a extension reaction with a polymerizing enzyme, and detecting the presence of extension products. The reaction extension mixture medium contains an intercalating agent such as ethidium bromide, dihydroethidium, ethidium homodimer-1, ethidium homodimer-2, acridine, or propidium iodide.
 

 
US Patent 7,312,052. Polymerase compositions and uses thereof.
Inventors: Joseph Sorge, Rebecca Mullinax.
Assignee: Stratagene (now Agilent Technologies).
 
Provides novel compositions containing a mixture of an enzyme that possesses substantial 3' 5' exonuclease activity and a DNA polymerase with less 3' 5' exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase and the Pfu DNA polymerase. Another aspect of the invention is to provide methods for synthesizing polynucleotides using similar compositions of polymerases.
 

 
US Patent 7,309,571. Amplification of self-ligated, circularized cDNA for expression profiling.
Inventor: John Nelson. 
Assignee: GE Healthcare Bio-Sciences.
 
Presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase-based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.
 

 
US Patent 7,303,876. Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
Inventors: Lawrence Greenfield, Luz Montesclaros.
Assignee: Applera.
 
The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples. In certain embodiments, the compositions include at least one cationic surfactant, at least one protease, and a buffer.

The Scan

Support for Moderna Booster

An FDA advisory committee supports authorizing a booster for Moderna's SARS-CoV-2 vaccine, CNN reports.

Testing at UK Lab Suspended

SARS-CoV-2 testing at a UK lab has been suspended following a number of false negative results.

J&J CSO to Step Down

The Wall Street Journal reports that Paul Stoffels will be stepping down as chief scientific officer at Johnson & Johnson by the end of the year.

Science Papers Present Proteo-Genomic Map of Human Health, Brain Tumor Target, Tool to Infer CNVs

In Science this week: gene-protein-disease map, epigenomic and transcriptomic approach highlights potential therapeutic target for gliomas, and more