Roche NimbleGen’s Sequence Capture Technology for enriching targeted genomic regions for high-throughput sequencing is now available for use in customers’ laboratories.
Researchers can order custom-designed Sequence Capture Arrays, along with equipment, reagents, and consumables. The company also offers customer workshops to gain hands-on experience with the technology. The entire DNA enrichment procedure takes approximately two weeks. The oligo microarrays carry 385,000 probes. Up to 5 megabases of contiguous or noncontiguous genomic sequence can be captured on a single array.
Roche also provides a new instrument, called Elution Station, which the company said will facilitate “consistent and reproducible capture results.” Roche NimbleGen said it is currently developing protocols that will allow the addition of 454 adapters into the sequence capture workflow.
GenBank release 167.0 is now available here via FTP from the National Center for Biotechnology Information. It contains data as of Aug. 19. The new release comprises approximately 95 gigabases and 92 million entries from non-WGS, non-CON sequences, and 118.6 gigabases and 40 million entries from WGS sequences.
Uncompressed, the 167.0 release flat files require about 357 GB for sequence files only. The ASN.1 data require approximately 326 GB.