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Sequence Capture 385K Arrays, GenBank 168.0, Imprint Methylated DNA Quantification Kit

Roche NimbleGen has launched an improved version of its Sequence Capture 385K arrays for enriching genomic regions for high-throughput sequencing, which the company developed in collaboration with the Human Genome Sequencing Center at Baylor College of Medicine. According to Roche NimbleGen, the workflow, starting from genomic DNA to sequencing results, takes about two weeks. Between 50 and 70 percent of reads map back to target regions, and the number of regions with low or high sequence coverage has been reduced. The company offers the new arrays both as custom products and as part of its sequence capture service.

GenBank release 168.0 is now available here via FTP from the National Center for Biotechnology Information. It contains data as of Oct. 27. The new release comprises approximately 97.4 gigabases and 96.4 million entries from non-WGS, non-CON sequences, and 136.1 gigabases and 46 million entries from WGS sequences.
Uncompressed, the 168.0 release flat files require about 371 GB for sequence files only. The ASN.1 data require approximately 338 GB.

Sigma-Aldrich has launched an Imprint Methylated DNA Quantification kit for epigenetic research. The kit employs an ELISA-like procedure to measure global DNA methylation in less than four hours. According to the company, the kit is effective with a DNA input as low as 5 nanograms of fully methylated DNA  Sigma-Aldrich licensed the MDQ technology from the Epigentek group.

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