Researchers Adapt RAD-Tagging to Next-Gen Sequencing, Improved Read Lengths | GenomeWeb

The fruits of a three-year effort have finally come to bear in the form of a new and improved sequencing method based on restriction-site associated DNA markers. A defining characteristic of a RAD tag is that one of its ends is fixed at a restriction site sequence while the other is sheared, allowing the surrounding genomic DNA to be sampled. Eric Johnson, an associate professor at the University of Oregon, originally developed the RAD marker method in 2005 when he applied it to microarrays; he has now expanded it to sequencing.

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