Q Chip of Cardiff, UK, has developed ReaX Assay PCR 16S beads to PCR-amplify 16S ribosomal RNA of bacteria. According to the company, setting up the reactions requires fewer pipetting steps and is quicker and more user-friendly to perform than conventional liquid-based PCR setup. The beads can also be used to amplify the rRNA gene directly from bacterial colonies, without requiring DNA isolation. ReaX Assay 16S beads contain all reagents required to perform 16S rRNA gene amplification, including a high-performance Taq polymerase, dNTPs, and the universal 16S rRNA gene-specific primers 27F and 907R. Users only need to add template DNA is required prior to running the PCR, and amplification is confirmed using end point PCR.
Epicentre Biotechnologies’ new Hybridase Thermostable RNase H specifically degrades the RNA in a DNA/RNA hybrid, leaving DNA or unhybridized RNA unaffected. Also, in contrast to standard E. coli RNase H, which is rapidly inactivated at 55°C, Hybridase RNase H is active at temperatures up to 95°C, allowing it to be used at temperatures that give the highest hybridization stringency. This maximizes sensitivity and selectivity while minimizing background, according to the company.
Epicentre also launched the ExactStart Full-Length cDNA Library Cloning Kit, which provides strong selection for cDNA clones derived from normal, full-length RNA. The kit allows for the identification of the precise 5’ and 3’ ends of both coding and non-coding transcripts and is well suited for mapping alternate transcription start sites, according to the company. The one-day process also clones many novel, noncoding RNAs, the firm claims.