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Papers of Note: Jul 19, 2011


A strategy for modular tagged high-throughput amplicon sequencing.
Aguirre de Cárcer D, Denman SE, McSweeney C, Morrison M.
Appl Environ Microbiol. 2011 Jul 15. [Epub ahead of print]

Scalable transcriptome preparation for massive parallel sequencing.
Stranneheim H, Werne B, Sherwood E, Lundeberg J.
PLoS One. 2011;6(7):e21910.

Application of microdroplet PCR for large-scale targeted bisulfite sequencing.
Komori HK, Lamere SA, Torkamani A, Hart GT, et al.
Genome Res. 2011 Jul 14. [Epub ahead of print]

Statistical mutation calling from sequenced overlapping DNA pools in TILLING experiments.
Missirian V, Comai L, Filkov V.
BMC Bioinformatics. 2011 Jul 14;12(1):287.

Bar-coding bias in high-throughput multiplex sequencing of miRNA.
Alon S, Vigneault F, Eminaga S, Christodoulou D, et al.
Genome Res. 2011 Jul 12. [Epub ahead of print]

A biologist's guide to de novo genome assembly using next-generation sequence data: A test with fungal genomes.
Haridas S, Breuill C, Bohlmann J, Hsiang T.
J Microbiol Methods. 2011 Jul 3. [Epub ahead of print]

Bonobos fall within the genomic variation of chimpanzees.
Fischer A, Prüfer K, Good JM, Halbwax M, et al.
PLoS One. 2011;6(6):e21605.

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.