NEW YORK (GenomeWeb News) – The National Heart, Lung and Blood Institute will give $20 million over the next five years to fund four sequencing centers and one data center that will study the microbes that reside in human lungs and air passages, including nasal and throat cavities, in individuals infected with HIV and in controls who are not infected.
These studies will aim to identify bacteria and other organisms such as viruses, protozoa, fungi, and organisms without cell walls.
NHLBI expects to fund the four clinical sequencing centers and a data collection center with up to $525,000 per year in direct costs for up to five years.
Investigators seeking to apply for funding should use high-throughput technology platforms to create data sets “of sufficient quality and depth” to allow for analysis of the microbes involved in the progression of HIV and in its complications, NHLBI said. The technologies that investigators use could include 167SrRNA gene sequencing and metagenomic shotgun sequencing.
These collected data will be used to study the impact of changes in the respiratory microbiome in the progression of HIV disease, HIV-related respiratory complications, and the effects of anti-HIV therapies.
The Clinical/Sequencing Centers will conduct individual projects, including harvesting and conducting analyses of the lung microbiome, and each must agree to share data and biospecimens and to cooperate with other centers and with the DCC.
The data collection center will support individual projects at each of the other sites, where it will help to develop and implement common protocols. It must be able to store and manipulate large data sets and to support quality control for collaborative protocols.
More information about the funding program is available here.