SoftGenetics has released a new version of its NextGENe software, which has improved SNP and indel detection capabilities. The new version includes a patent-pending condensation tool that “statistically polishes the data” by removing chemistry- and instrument-related errors, sorting the raw data by a 12-base anchor sequence and sequence shoulders, and assembling it by further condensation cycles, according to the company. The software can use data from Illumina’s Genome Analyzer, 454 Life Sciences, and Applied Biosystems’ SOLiD platform. NextGENe analysis modules include SNP/indel detection, de novo and target assembly, digital gene expression analysis, transcriptome analysis, ChipSeq analysis, and miRNA discovery and quantification. Interested parties can request a free 30-day evaluation or schedule a web-based review meeting.
Epicentre Biotechnologies has released its End-It DNA end repair kit for treating fragmented genomic DNA for use in next-generation DNA sequencing. The kit converts DNA containing damaged or 5′ and/or 3′ protruding ends to 5′-phosphorylated, blunt-ended DNA for ligation to sequencing adaptors. The kit contains reagents for 20 end-repair reactions, up to 100 micrograms of genomic DNA. The kit can be combined with Epicentre’s Fast-Link DNA ligation kit.
Sigma-Aldrich has launched its GenElute HP 96-well plasmid miniprep kit for DNA purification. The kit allows for rapid purification of plasmid DNA to transfection-grade quality. Up to 10 micrograms of high-copy plasmid DNA can be recovered per well from 1.3 milliliters of harvested bacterial culture in less than 50 minutes, according to the company. The kit is compatible with automated liquid handling workstations and offers a vacuum manifold-based manual protocol.