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Next-generation sequencing software suite, REPLI-g FFPE

SoftGenetics will preview a suite of software analysis tools for next-generation sequencing systems at the Association of Biomolecular Resource Facilities annual meeting in Salt Lake City next month. The software suite contains SNP mutation analysis/expression, target sequence, and de novo assembly modules for data generated by Illumina’s Genome Analyzer, 454’s Genome Sequencer FLX, and Applied Biosystems’s SOLiD sequencing system. Interested researchers not attending ABRF can contact SoftGenetics directly to arrange for an on-line demonstration of the software suite.

Qiagen has launched the REPLI-g FFPE kit for whole-genome amplification. The kit uses a novel DNA processing reaction, which allows multiple displacement amplification, or MDA, to be extended to highly degraded DNA samples derived from formalin-fixed, paraffin-embedded tissue. Isolating genomic DNA from such tissues is often difficult, according to the company, because very little DNA is available. The new kit allows researchers to amplify DNA while maintaining locus representation. Uniform whole genome amplification directly from FFPE tissue can be performed without the need for prior DNA isolation. A two-hour amplification reaction typically yields 10 µg DNA, whereas 40 µg can be obtained after eight hours of incubation. Once amplified, the DNA is suitable for use in most downstream genotyping assays without further purification.

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