Thermo Fisher Scientific has made its Thermo Scientific Thermo-Start range of hot start PCR reagents available with ReddyMix buffer.
According to the company, the buffer helps with an easier, error-free set-up and improves reproducibility of the reactions. It contains an inert red dye, which enhances the contrast between the reagents and the plastic vessel, so that users can verify accurate pipetting. The red dye and precipitant contained in the buffer also helps users to load final PCR products directly onto agarose gels.
According to the company, Thermo-Start reagents are well suited for the amplification of complex genomic or cDNA templates and for low copy-number targets, such as single cell PCR. The reagents are also available with a high-performance buffer for sensitive and specific amplification of challenging targets, such as highly complex and GC-rich templates.
Golden Helix has launched SNP & Variation Suite version 7, a software suite for the analysis of single nucleotide polymorphisms and copy number variations. According to the company, SVS 7 was designed to handle large datasets and to provide a framework to make the analysis easier and possible on a desktop computer.
GenBank release 170.0 is now available here via FTP from the National Center for Biotechnology Information. It contains data as of Feb. 13. The new release comprises approximately 101 gigabases and 101 million entries for non-WGS, non-CON sequences, and 143 gigabases and 49 million entries for WGS sequences.
Uncompressed, the 170.0 release flat files require about 390 GB for sequence files only. The ASN.1 data require approximately 356 GB.