New England Biolabs has launched NEBNext, a series of reagents that facilitate sample preparation for next-generation sequencing, expression library construction, and other applications. Reagents, which are sold as sets, master mixes, or modules, are available for each step of the library construction process, including DNA end repair, dA-tailing, adapter ligation, and DNA amplification. In addition, the company offers NEBNext dsDNA Fragmentase for fragmenting DNA enzymatically.
Agilent Technologies has introduced a SureSelect Human All Exon Kit for target enrichment prior to next-generation DNA sequencing. The product will be available to customers next month in sizes appropriate for studies with a few to several thousand samples. The kit design covers 1.22 percent of human genomic regions, corresponding to the NCBI Consensus CDS Database and includes more than 700 human miRNAs from the Sanger v13 database and more than 300 additional human non-coding RNAs. The kit will initially be available for use with paired-end sequencing on the Illumina Genome Analyzer II. A kit version that is optimized for the Applied Biosystems SOLiD platform is in the final stages of optimization and will follow shortly, according to Agilent.
Olink Genomics of Uppsala, Sweden, has launched a targeted resequencing service that uses its Selector technology for enriching regions of the genome. According to the company, the service amplifies thousands of fragments in a single tube.
GenomeQuest is offering genomics researchers a free basic account to its GenomeQuest 6.0Beta sequence data management product, which allows scientists to manage, mine, and share next-generation sequence data and results and access a collection of sequence reference databases. The free account gives researchers access to all GenomeQuest 6.0Beta functionality and complete results of Sanger sequence runs, as well as a sampling of next-generation sequencing run results. Access to all results of next-generation sequencing runs is available for an annual subscription that starts at $1,500.