GE Healthcare has launched Illustra TriplePrep, a kit to isolate genomic DNA, RNA, and total denatured protein simultaneously from a single, undivided sample. The kit contains two spin columns and six buffers for use with mammalian cell culture or animal tissue, and the preparation takes one hour, according to the company. The isolated gDNA, total RNA, and total denatured proteins are suitable for a range of applications, including PCR, restriction enzyme digestion, sequencing, array CGH, RT-PCR, gene expression microarrays, SDS-PAGE, Western blotting, 2-D DIGE, and LCMS.
SoftGenetics has added a SNP and indel detection module for reads from the Roche/454 Genome Sequencer FLX to its NextGene software.
The new module addresses the homopolymer-based errors of the FLX system. It uses high coverage of reads "to statistically polish and correct the inherent system errors," according to the company. Also, NextGene's alignment tool permits base pair mismatches between the sample sequences and reference, allowing the software to accurately align reads with long indels and identify them as mutations. The SNP detection application for 454 data identifies SNPs by accurately aligning sample reads with a reference.
Interested parties may request a 30-day trial of the software, or arrange for a web-based demonstration using their own data at the company's web site.