NEW YORK – New long-read sequencing-based methods for analyzing RNA isoforms are proving a valuable complement to short-read sequencing.
Using sequencing platforms from Pacific Biosciences and Oxford Nanopore Technologies, researchers are able to more precisely analyze RNAs thousands of bases long and their effect on gene transcription, translation into proteins, and ultimately cellular function. Applications include single-cell studies, analysis of exotic RNA species, and even research on RNA viruses, including SARS-CoV-2.