GenBank release 165.0 is now available here via FTP from the National Center for Biotechnology Information. It contains data as of April 15. The new release contains approximately 89 billion base pairs and 86 million entries from non-WGS and non-CON sequences, and 110 billion base pairs and 27 million entries from WGS sequences. Uncompressed, the 165.0 release flat files require about 332 GB for sequence files only. The ASN.1 data require approximately 305 GB.
A new GenBank division, called Transcriptome Shotgun Assembly, or TSA, has become legal with the 165.0 release. TSA sequences are shotgun assemblies of primary sequences deposited in dbEST, the Trace Archive, or the Short-Read Archive. No format changes are anticipated for this new class of GenBank record, NCBI said.
Sigma-Aldrich has launched the Imprint Chromatin Immunoprecipitation Kit for epigenetic research. The kit contains columns and reagents for DNA purification as well as a protocol for ChIP DNA amplification with the GenomePlex whole-genome amplification kit. It allows for immunoprecipitation and purification of DNA from mammalian cells or tissues in a microwell format. The kit is based on Chromatin IP technology licensed from the Epigentek Group.