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Applied Biosystems: Feb 27, 2007

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Applied Biosystems has launched the BigDye XTerminator purification kit, which helps with the 'clean-up' step in Sanger sequencing.
 
According to the company, the new purification kit is designed to shorten the amount of time and work required to perform the purification step, and to do so in a manner that is more reliable than other methods.
 
The kit involves a 4-step protocol that is said to provide more reliable dye blob removal, increased sample stability, and more reproducible, higher-quality results. The kit consists of two reagents, the XTerminator solution, which binds impurities but leaves DNA in solution, and the SAM solution, an additive that enables complete removal of unincorporated terminators and stabilizes sequenced DNA fragments.
 
The product is designed to work in conjunction with the Applied Biosystems BigDye terminator sequencing chemistry, which is used to amplify and create fluorescent dye-labeled terminated fragments for automated sequencing analysis.

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