454 Targets Longer Reads, Better De Novo Assembly; Cites Clinical Apps of Amplicon Sequencing | GenomeWeb

Roche's 454 Life Sciences continues to develop longer reads for its Genome Sequencer FLX system and to improve its sample-prep process, multiplex identifying tags, and paired-end library protocols, according to a company official.

In addition, the company has developed an Amplicon Variant Analyzer software for "ultra-deep" amplicon sequencing — an application that it says is increasingly used for clinical sequencing projects — and has further developed its Newbler assembler in order to be able to assemble large eukaryotic genomes de novo.

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