Skip to main content
Premium Trial:

Request an Annual Quote

This Week in PNAS: Dec 9, 2014

Editor's Note: Some of the articles described below are not yet available at the PNAS site, but they are scheduled to be posted some time this week.

In the early, online edition of the Proceedings of the National Academy of Sciences, researchers from the University of Michigan and the University of Maryland describe fitness genes turned in uropathogenic Escherichia coli — a form of E. coli implicated in urinary tract infections — as it infects its host. The team used RNA sequencing to profile transcriptional patterns in uropathogenic E. coli found in urine samples from individuals with acute, uncomplicated UTIs. Among the genes up-regulated in the bug's transcriptional signature were ion transport-coding genes suspected of bolstering its survival during human infection.

A team from Russia, the UK, and the US used scoured enzyme coding genes in more than a dozen plant genomes to begin tallying up the diversity found in a group of plant natural products known as terpenes. Based on terpenoid synthase and cytochrome P450 gene repertoires in nine eudicot plan genomes, four monocot plant genomes, and a handful of additional dicot and monocot scaffold sequences, the researchers saw slight genomic organization of the terpene metabolism coding components in the eudicot and monocot plant groups. "Our analyses shed light on the roots of terpene biosynthesis and diversification in plants," the study's authors say. "They also reveal that different genomic mechanisms of pathway assembly predominate in eudicots and monocots."

Finally, New Zealand researchers report on an optogenetic system that they developed for studying populations of neurons known as gonadotropin-releasing hormone neurons, which are distributed in small clusters throughout the mammalian brain and contribute to hormone pulses involved in reproduction and other functions. The method involved transfecting such neurons with light-activated channelrhodopsin proteins in mice, they say, producing a system that selectively activates the neurons of interest of stretches of between 30 seconds and five minutes.

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.