A team led by investigators at the Iowa State University and Data2Bio present a genotyping-by-sequencing method designed to pick up heterozygous SNPs and other genotyping information that may be missed by typical genotyping-by-sequencing methods. The genome reduction-based approach, known as tGBS, relies on double-digestion with two restriction enzymes and replaces double-stranded adaptor molecules with single-stranded oligonucleotides, the researchers explain.

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