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In Nature Biotechnology this week, a Seoul National University team reports a new CRISPR-based method for assessing the genome-wide target specificity of adenine base editors. The scientists created a modified version of Digenome-seq — an in vitro whole-genome sequencing method for identifying CRISPR-induced double-strand breaks — that could detect adenine base editor off-target sites with a substitution frequency of 0.1 percent or more, they write.

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