In the Proceedings of the National Academy of Sciences, investigators at Xiamen University, Chongqing Medical University, and other centers in China outline an automatic digital microfluidics-based method for profiling copy number variants (CNVs) in individual cells — an approach they applied to individual cells from cell line, amniotic fluid, and peripheral blood sample sources. The digital counting and microfluidics approach, known as dd-scCNV Seq, relies on a so-called "digital microfluidics for digital counting of single-cell copy number variation" library preparation procedure, the team notes, which involves direct fragmentation and amplification on a digital microfluidics chip containing an array of 48 electrodes. "Instead of relying on read depths, dd-scCNV Seq achieves digital counting of copy numbers due to single molecule amplification of these unique segments," the authors write, adding that dd-scCNV Seq "shortens the time from days to hour for generation of barcoded single-cell DNA libraries with a mean size suitable for [next-generation sequencing]."
Single-Cell CNV Profiling Performed With Digital Microfluidics Method
May 09, 2023