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Nucleic Acids Research Paper Presents Single-Cell Sequencing Method for De Novo Assembly

A team of Peking University investigators describes a sequencing protocol for doing de novo genome assembly using single-cell long-read sequencing data. As the team reports in the journal Nucleic Acids Research, it turned to its "single-molecule, real-time sequencing of long fragments amplified through transposon insertion" (SMOOTH-seq) method to help in putting together high-continuity genome assemblies with single-cell whole-genome sequence data generated for a few dozen individual human cells with Pacific Biosciences or Oxford Nanopore sequencing platforms. "[W]e have employed SMOOTH-seq on PacBio HiFi and Oxford Nanopore Technologies platforms to sequence K562 (a human chronic myelogenous leukemia (CML) cell line and HG002 (a normal diploid lymphoblast cell line)," the team writes, "and demonstrate the feasibility of genome assembly based on scWGS dataset with different assemblers and rigorous evaluations."

The Scan

ChatGPT Does As Well As Humans Answering Genetics Questions, Study Finds

Researchers in the European Journal of Human Genetics had ChatGPT answer genetics-related questions, finding it was about 68 percent accurate, but sometimes gave different answers to the same question.

Sequencing Analysis Examines Gene Regulatory Networks of Honeybee Soldier, Forager Brains

Researchers in Nature Ecology & Evolution find gene regulatory network differences between soldiers and foragers, suggesting bees can take on either role.

Analysis of Ashkenazi Jewish Cohort Uncovers New Genetic Loci Linked to Alzheimer's Disease

The study in Alzheimer's & Dementia highlighted known genes, but also novel ones with biological ties to Alzheimer's disease.

Tara Pacific Expedition Project Team Finds High Diversity Within Coral Reef Microbiome

In papers appearing in Nature Communications and elsewhere, the team reports on findings from the two-year excursion examining coral reefs.