Title: snoRNAi-Small Nucleolar RNA Degradation by RNA Interference in Trypanosomatids
Filed: Aug. 2, 2005
Inventor: Shulamit Michaeli, Bar-LLan University
According to the patent application's abstract, the invention comprises "polynucleotides and a method suitable for downregulation of small nuclear RNA which can be used to treat diseases associated with activity of small nuclear RNA. Specifically, the … invention can be used to downregulate snoRNA molecules or box H/ACA-containing RNA molecules which are involved in diseases such as cancer."
Title: Two-Color Real-Time/End-Point Quantitation of microRNAs
Filed: Sept. 21, 2005
Lead Inventor: Andrew Finn, Applera
The invention is "directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples," the patent application's abstract states. "A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions."
Title: Methods and Compositions for RNA Interference
Filed: April 14, 2005
Lead Inventor: Michaeline Bunting, Invitrogen
According to the patent application's abstract, the invention "provides methods and compositions related to the field of gene expression regulation. In particular, methods and compositions of the invention can be used to identify RNAi cleavage sites along a target RNA molecule. Methods and compositions of the invention may also be used to knockdown expression of nucleic acid molecules which encode reporters."