Title: Method for Generation of a Random RNAi Library and its Application in Cell-Based Screens. Number: 20040152172. Filed: Sept. 5, 2003. Inventor: Martin Geppert, Pharmacia & Upjohn (Pfizer).
“The present invention provides methods and compositions relating to inhibitory RNA,” the patent application’s abstract states.
The application specifically claims “a method to generate a population of inhibitor sequences ready for cloning [by] extending a population of random oligonucleotide RNAi progenitors comprising a fixed primer sequence, a random oligonucleotide sequence, and a fixed stem-loop structure, via a polymerase extension reaction to produce a full hairpin random oligonucleotide RNAi progenitor.” The method described further involved “denaturing said full hairpin random oligonucleotide RNAi progenitor to produce a denatured full hairpin random oligonucleotide RNAi progenitor; extending said denatured full hairpin random oligonucleotide RNAi progenitor via a polymerase extension reaction to create a double stranded linear product; and removing primer sequences from said double stranded product,” the application states.
Title: Deprotection of RNA. Number: 20040147735. Filed: Jan. 6. 2004. Lead Inventor: Laurent Bellon, Sirna Therapeutics.
The invention is a method for one-pot deprotection of RNA molecules, the patent application’s abstract states.