By designing its siRNAs using the algorithm, Sigma-Aldrich said it guarantees that two out of three siRNA duplexes per target gene will achieve knockdown efficiencies of greater than or equal to 75 percent.
Qiagen has recently launched the miScript System, which allows for the detection and quantification of miRNA, as well as mRNA and other small RNA, using SYBR Green-based rtPCR.
With the system, researchers can quantify multiple miRNAs from a single cDNA synthesis reaction, Qiagen said. Both miRNA and mRNA can be quantified from the same cDNA synthesis reaction, the company added.
Sigma-Aldrich said that its Mission siRNA libraries are now incorporating Rosetta Inpharmatics’ siRNA design algorithm.