Qiagen has just released two protocols for use with the company's RNeasy kits.
The first protocol provides microRNA purification from a range of sample types, including total RNA, cultured cells, soft animal tissues, and plant tissues, said Qiagen. The protocol is for use with the RNeasy mini-kit in combination with the RNeasy MinElute Cleanup kit, and allows either co-purification or separate purification of microRNA and total RNA greater than 200 nucleotides.
The second protocol allows for the purification of microRNA from all animal and plant tissues, said Qiagen. Both the RNeasy Lipid Tissue mini-kit and the RNeasy MinElute Cleanup kit are required, the company added.
Additional details about the protocols can be found at www.qiagen.com/goto/microRNAprotocols.
Invitrogen is offering a 50 percent discount on select nucleic acid purification kits.
According to the company, customers can receive 50 percent off their first purchase of certain of the company's new kits, including its PureLink miRNA isolation kit and its FastTrack MAG mRNA isolation kits.
Additional details about the offer, which runs through March 31, can be found at http://www.invitrogen.com/content.cfm?pageid=10577&CMP=ILC-NAPROMO
Ambion has just introduced the FlashPage fractionator, which is used for the purification of small nucleic acids.
According to the company, the fractionator is a miniaturized electrophoresis instrument designed for rapid PAGE-purification of small nucleic acids with an average run time of 12 minutes.
"Fractionation occurs with the passage of the nucleic acids through a proprietary denaturing gel matrix into a lower buffer collection chamber," Ambion said. "The denaturing gel matrix is supplied in the form of convenient pre-cast gel cartridges. The lower buffer chamber has been designed for retention of eluted material to facilitate subsequent nucleic acid purification.
Additional details can be found at http://www.ambion.com/catalog/CatNum.php?13100.