GeneCopoeia has recently launched a collection of microRNA functional analysis reagents and tools.
These include miExpress vector-based precursor miRNA-expression clones, which cover all known human, mouse, and rat miRNAs; miTarget miRNA target sequence-expression clones, which cover genome-wide human, mouse and rat genes; and the All-in-One miRNA RT-qPCR system, which consists of a reverse transcription kit, a SYBR green RT-qPCR master mix kit, and a complete set of validated primers for human, mouse, and rat miRNAs.
Sigma-Aldrich has introduced the Mission LentiPlex pooled human and murine shRNA libraries.
These collections include a U6 promoter for shRNA expression in a wide variety of cell lines including embryonic stem cells, the company noted.
Promega has introduced the pmirGlo dual-luciferase miRNA target-expression vector, which the company said allows a luciferase reporter gene assay to measure the function of endogenous or exogenous miRNAs.
"This vector also enables functional studies of sequence variations found in 3'-untranslated regions of genes often related to miRNA binding sites or other modulators of transcript stability," Promega noted.