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Kreatech Biotechnology s Universal Linkage System, Ambion s Melt Total RNA Isolation System, and Promega s pGL4 Luciferase Reporter Vectors

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Kreatech Biotechnology said this week that it has launched labeling kits for the DNA microarray market using the company’s Universal Linkage System labeling technology.

The ULS technology, said the company, relies on the special binding properties of transition metals to form coordinative bonds with biomolecules by binding to specific sites on DNA, RNA, and proteins.

Complete labeling kits include purification columns, blocking buffer, hybridization buffers, and ULS bound with the most common fluorescent dye pairs, including dyes from GE Healthcare, Invitrogen, PerkinElmer, and Dyomics.


Ambion has recently introduced the Melt Total RNA isolation system.

According to the company, the system is an alternative to traditional RNA isolation procedures that is also amenable to high-throughput tissue processing. Samples can be lysed quickly and easily in a closed-tube format that minimizes cross-contamination, said Ambion.

The system allows for the rapid digestion of fresh or frozen tissue, the company said. Following digestion, the RNA is purified using a streamlined process based on Ambion’s MagMax magnetic bead procedure.

Additional details about the system can be found at http://www.ambion.com/catalog/CatNum.php?1982.


Promega has introduced its pGL4 luciferase reporter vectors.

According to the company, the reporter gene vectors are optimized for expression in mammalian cells, and come in numerous configurations, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes.

Both the reporter genes and the vector backbone have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription, Promega said.

Additional details about the vectors can be found at http://www.promega.com/tbs/tm259/tm259.html.

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