Title: Method of Diagnosing Acute Lymphomic Leukemia Using miR-146a
Patent Number: 8,372,586
Filed: Oct. 17, 2011
Inventor: Carlo Croce, Ohio State University
The invention, the patent's abstract states, comprises “compositions and methods for reducing the proliferation of ALL cancer cells through targeted interactions with ALL1 fusion proteins.”
Title: Methods and Compositions for the Specific Inhibition of KRAS by Asymmetric Double-Stranded RNA
Patent Number: 8,372,816
Filed: April 5, 2010
Inventor: Bob Brown, Dicerna Pharmaceuticals
“This invention relates to compounds, compositions, and methods useful for reducing KRAS target RNA and protein levels via use of Dicer-substrate siRNA agents possessing asymmetric end structures,” the patent's abstract states.
Title: Small Interfering RNA for Gene Knockdown of the Subcutaneous N-Methyl-D-Aspartate Receptor NR1 Subunit and Its Application on Pharmaceutics
Patent Number: 8,372,817
Filed: May 14, 2010
Inventor: Ping-Heng Tan, I-Shou University
The patent, its abstract states, claims “a small interfering RNA for gene knockdown of the N-methyl-D-aspartate receptor NR1 subunit … [as well as a] method of using the small interfering RNA [by] applying the small interfering RNA on subcutaneous tissues [to temporarily] interfere with the genetic expression of the NMDA receptor NR1 subunit in [the] hypoderm.” Also claimed is the use of the siRNA to treat inflammatory or chronic pain.
Title: RNA Interference-Mediating Small RNA Molecules
Patent Number: 8,372,968
Filed: Aug. 7, 2009
Lead Inventor: Thomas Tuschl, Mack Planck Institute
“Double-stranded RNA induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference,” the patent's abstract states. “Using a Drosophila in vitro system, we demonstrate that 19-23 [nucleotide] short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.”
Title: RNA Interference Methods Using DNA-RNA Duplex Constructs
Patent Number: 8,372,969
Filed: Oct. 25, 2010
Lead Inventor: Shao-Yao Ying, University of Southern California
The invention, the patent's abstract states, “provides novel compositions and methods for suppressing the function or activity of a targeted gene through a novel intracellular piRNA-mediated RNAi mechanism using RNA-DNA duplex constructs. The invention further provides novel methods and compositions for generating or producing RNA-DNA duplex agents, whose quantity is high enough to be used for the invention's gene silencing transfection and possibly in therapeutic applications. This improved RNA-polymerase chain reaction method utilizes thermocycling steps of promoter-linked DNA or RNA template synthesis, in vitro transcription, and then reverse transcription to bring up the amount of RNA-DNA duplexes up to two thousand folds within one round of the above procedure for using in D-RNAi-directed gene silencing.”
Title: Oligonucleotide Compounds Comprising Non-Nucleotide Overhangs
Application Number: 20130035368
Filed: Jan. 6, 2011
Lead Inventor: Sharon Avkin-Nachum, Quark Pharmaceuticals
“The invention relates to siRNA compounds comprising one non-nucleotide moiety covalently attached to at least one of the sense or antisense strands to down-regulate the expression of human genes,” according to the patent application's abstract. “The invention also relates to pharmaceutical compositions comprising such compounds and a pharmaceutically acceptable carrier and to methods of treating and/or preventing the incidence or severity of various diseases or conditions associated with the target genes and/or symptoms associated with such diseases or conditions.”
Title: Double-Stranded Compositions Comprising Differentially Modified Strands for use in Gene Modulation
Application Number: 20130035369
Filed: Aug. 3, 2012
Lead Inventor: Balkrishen Bhat, Isis Pharmaceuticals
The invention, the patent application's abstract states, provides “double-stranded compositions wherein the first strand is modified to have a particular motif and the second strand is a modified selected motif. More particularly, the ... compositions comprise an antisense strand that is modified to have a positional/full motif and the sense strand is modified to have an alternating motif, a hemimer motif, a blockmer motif, a gapped motif, a positional motif, a positional/full motif, or a fully modified motif. Each strand further comprises one or more phosphorothioate internucleoside linkage[s]. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes.”
Title: Lipid-Formulated dsRNA Targeting the PCSK9 Gene
Application Number: 20130035371
Filed: Aug. 7, 2012
Lead Inventor: Kevin Fitzgerald, Alnylam Pharmaceuticals
“This invention relates to composition and methods using lipid formulated siRNA targeted to a PCSK9 gene,” the patent application's abstract states.
Title: microRNA Induction of Pluripotent Stem Cells and Uses Thereof
Application Number: 20130035374
Filed: March 25, 2011
Inventor: Edward Morrisey, University of Pennsylvania
The invention, the patent application's abstract states, relates to “compositions and methods for inducing the formation of an induced pluripotential stem cell from a somatic cell. … The compositions comprise miR 302-367 cluster and valproic acid. Further disclosed are methods for treatment of a disease or condition in a subject through the use of the compositions.”