Title: Method and Compositions Involving microRNA
Patent Number: 8,465,914
Filed: July 2, 2012
Lead Inventor: David Brown, Asuragen
The invention concerns “methods and compositions for isolating, enriching, and/or labeling miRNA molecules, and for preparing and using arrays or other detection techniques for miRNA analysis,” the patent’s abstract states. “Moreover, the … invention concerns methods and compositions for generating miRNA profiles and employing such profiles for therapeutic, diagnostic, and prognostic applications.”
Title: Ultraconserved Regions Encoding ncRNAs
Patent Number: 8,465,918
Filed: Aug. 4, 2008
Inventor: Carlo Croce, Ohio State University
The patent, its abstract states, claims “methods for [differentiating] human cancers … using one or more transcribed ultraconserved regions expression profiles where the association between the genomic location of UCRs and the analyzed cancer-related genomic elements is highly statistically significant and comparable to that reported for miRNAs.”
Title: Compositions and Methods for Modulating Angiogenesis
Patent Number: 8,466,117
Filed: July 30, 2007
Lead Inventor: Joshua Mendell, Johns Hopkins University
The invention, the patent’s abstract states, features “compositions and methods … for modulating angiogenesis.” Specifically, the patent claims the use of microRNA inhibitors to modulate angiogenesis.
Title: Methods and Compositions for Inducing Deregulation of EPHA7 and ERK Phosphorylation in Human Acute Leukemias
Patent Number: 8,466,119
Filed: Aug. 22, 2008
Inventor: Carlo Croce, Ohio State University
The invention, the patent’s abstract states, comprises “methods for assessing a pathological condition in a subject [including by] measuring one or more markers where a difference is indicative of acute lymphoblastic leukemia or a predisposition to ALL.” The patent also claims the use of siRNAs that interfere with the ALL response-signaling pathway.
Title: Oligomeric Compound and Compositions for Use in Modulation of Pri-miRNAs
Patent Number: 8,466,120
Filed: June 10, 2010
Lead Inventor: Bridget Lollo, Regulus Therapeutics
The invention, the patent’s abstract states, comprises “compounds, compositions, and methods … for modulating the levels expression, processing, and function of pri-miRNAs. In particular, methods and compounds are provided for the modulation of the levels, expression, processing, or function of polycistronic pri-miRNAs. The compositions comprise oligomeric compounds targeted to small non-coding RNAs and pri-miRNAs. Further provided are methods for selectively modulating pri-miRNA levels in a cell. Also provided are methods for identifying oligomeric compounds that result in increased pri-miRNA levels when contacted with a cell.”
Title: Trialkyl Cationic Lipids and Methods of Use Thereof
Patent Number: 8,466,122
Filed: Sept. 16, 2011
Lead Inventor: James Heyes, Protiva Biotherapeutics (Tekmira Pharmaceuticals)
The invention, the patent’s abstract states, comprises “compositions and methods for the delivery of therapeutic agents to cells. In particular, these include novel cationic lipids and nucleic acid-lipid particles that provide efficient encapsulation of nucleic acids and efficient delivery of the encapsulated nucleic acid to cells in vivo. The compositions of the … invention are highly potent, thereby allowing effective knock-down of a specific target protein at relatively low doses. In addition, the compositions and methods of the … invention are less toxic and provide a greater therapeutic index compared to compositions and methods previously known in the art.”
Title: Hepatitis C Virus Inhibitors
Patent Number: 8,466,273
Filed: Sept. 30, 2008
Lead Inventor: Kyoko Kohara, Chemo-Sero-Therapeutic Research Institute
The invention relates to “hepatitis C virus inhibitors capable of inhibiting viral replication in hepatitis C virus-infected cells,” the patent’s abstract states. “The replication of hepatitis C virus can be inhibited and hepatitis C virus-infected cells can be specifically injured by … inhibiting BGT-1 or AKR1C1 involved in the replication of hepatitis C virus. Thus, viral inhibitors comprising a substance inhibiting BGT-1 or AKR1C1 are effective for the treatment of hepatitis C.”
The patent also claims the use of siRNAs against BGT-1 and AKR1C1.
Title: RNAi Therapeutic for Treatment of Hepatitis C Infection
Patent Number: 8,466,274
Filed: Oct. 2, 2012
Inventor: Hengli Tang, Florida State University
The invention relates to “small interfering RNAs or small hairpin RNA and compositions comprising same … that target human cyclophilin A to inhibit hepatitis C infection,” according to the patent’s abstract. Also claimed are “DNA polynucleotides, plasmids, and viral or non-viral vectors … that encode siRNA or shRNA molecules, which may be delivered directly to cells or in combination with delivery agents, such as lipids, polymers, encapsulated lipid particles, such as liposomes. Methods for treating, managing inhibiting, preventing, etc., HCV infection using such siRNA and shRNAs and compositions comprising same are also provided.”
Title: Novel microRNAs for the Detection and Isolation of Human Embryonic Stem Cell-derived Cardiac Cell Types
Application Number: 20130150256
Filed: June 14, 2011
Lead Inventor: Jane Synnergren, University of Skovde
The invention, the patent application’s abstract states, relates to “the use of human-derived microRNAs as targets for the identification of cardiac and cardiac-like cell types. In particular, it relates to a specific set of miRNAs which have been found to be correlated to cardiac differentiation and can act to up- or downregulate a number of putative mRNA targets to guide differentiation and also act as markers for a cardiac phenotype. In addition, it also relates to the use of these miRNAs as tools for the isolation, selection, purification, and characterization of cardiac and cardiac-like cells and tissues. The invention also encompasses the possible use of these miRNAs in the differentiation and maturation of cardiac or cardiac-like cell types.”
Title: Recombinant Protein for Intracellular Delivery of siRNA and Composition Comprising the Same
Application Number: 20130150287
Filed: Dec. 7, 2011
Lead Inventor: Hyung-Jun Ahn, Korea Institute of Science and Technology
The invention, the patent application’s abstract states, relates to “a recombinant protein for siRNA delivery, which allows the efficient intracellular and in vivo delivery of siRNA. More particularly, the … invention relates to a recombinant protein that allows a siRNA binding protein to be located in the interior cavity of a capsid protein of hepatitis B virus, in which siRNAs of interest bind to the siRNA binding protein to be encapsulated within the capsid shell, thereby providing stability against the external attack such as nucleases and achieving the efficient intracellular and in vivo delivery of siRNA by its release into the cytosolic space after cell uptake.”
Title: Methods for Impairing the p53/HDM2 Auto-Regulatory Loop in Multiple Myeloma Development using miR-192, miR-194, and miR-215
Application Number: 20130150430
Filed: Aug. 4, 2011
Lead Inventor: Carlo Croce, Ohio State University
The patent application, its abstract states, claims “methods and compositions for detecting, treating, characterizing, and diagnosing multiple myeloma.”
Title: RNA Interference-mediated Inhibition of Hepatitis B Virus Gene Expression Using Short Interfering Nucleic Acid
Application Number: 20130150433
Filed: Aug. 12, 2011
Lead Inventor: Steve Bartz, Merck
The invention, the patent application’s abstract states, “relates to compounds, compositions, and methods for the study, diagnosis, and treatment of traits, diseases, and conditions that respond to the modulation of HBV gene expression and/or activity, and/or modulate a HBV gene expression pathway. Specifically, the invention relates to double-stranded nucleic acid … that are capable of mediating or that mediate RNA interference against HBV gene expression.”
Title: Nuclease-resistant Double-stranded Ribonucleic Acid
Application Number: 20130150570
Filed: March 27, 2012
Lead Inventor: Hans-Peter Vornlocher, Alnylam Pharmaceuticals
“This invention relates to modified double-stranded oligoribonucleic acid having improved stability in cells and biological fluids, and methods of making and identifying dsRNA having improved stability, and of using the dsRNA to inhibit the expression or function of a target gene,” the patent application’s abstract states.