Skip to main content
Premium Trial:

Request an Annual Quote

Dana-Farber, Broad Team Uses shRNA Screen to ID Ovarian Cancer-Related Gene


Researchers from the Dana-Farber Cancer Institute, in collaboration with the Broad Institute, published research this week showing that a genome-scale shRNA study of more than 100 cancer cell lines revealed previously unknown lineage-specific dependencies in ovarian cancer.

The findings appeared in the Proceedings of the National Academy of Sciences.

The researchers used a pool of more than 54,000 shRNAs contained in a library developed by the Broad's RNAi Consortium (GSN 8/5/2010) to study the cell lines, which included 25 ovarian cancer tumors. The work revealed 54 genes that are essential for ovarian cancer proliferation and viability.

One gene in particular, PAX8, was found to be amplified in 16 percent of high-grade serious ovarian cancers, and expressed at higher levels in ovarian tumors, according to the paper.

“Suppression of PAX8 selectively induces apoptotic cell death of ovarian cancer cells,” the study's authors wrote.

In addition to identifying PAX8 as an ovarian lineage-specific dependency, the findings demonstrate that “the integration of genome-scale functional and structural studies provides an efficient path to identify dependencies of specific cancer types on particular genes and pathways,” the authors added.

The Scan

Researchers Compare WGS, Exome Sequencing-Based Mendelian Disease Diagnosis

Investigators find a diagnostic edge for whole-genome sequencing, while highlighting the cost advantages and improving diagnostic rate of exome sequencing in EJHG.

Researchers Retrace Key Mutations in Reassorted H1N1 Swine Flu Virus With Avian-Like Features

Mutations in the acidic polymerase-coding gene boost the pathogenicity and transmissibility of Eurasian avian-like H1N1 swine influenza viruses, a PNAS paper finds.

Genome Sequences Reveal Evolutionary History of South America's Canids

An analysis in PNAS of South American canid species' genomes offers a look at their evolutionary history, as well as their relationships and adaptations.

Lung Cancer Response to Checkpoint Inhibitors Reflected in Circulating Tumor DNA

In non-small cell lung cancer patients, researchers find in JCO Precision Oncology that survival benefits after immune checkpoint blockade coincide with a dip in ctDNA levels.