Skip to main content
Premium Trial:

Request an Annual Quote

Ambion siRNA libraries, Oligonucleotides, System Biosciences, and GeneNet Lentiviral siRNA Transduction System

Premium

Ambion said last week that it has launched two new siRNA libraries. The first targets 597 human kinases and the second targets 441 human G protein-coupled receptors.

According to the company, the kinase library includes one or more functionally validated siRNAs, which have been proven to effici- ently decrease levels of mRNA, targeting 231 kinases.

The siRNAs, Ambion added, were designed using an algorithm provided by partner Cenix BioScience.


Dharmacon said this week that it has launched its siStable siRNA, a proprietary form of siRNA modified to enhance stability, potency, and silencing longevity while decreasing toxicity.

According to the company, siStable siRNAs maintained full silencing potency after seven days in cell culture studies. In human serum, Dharmacon added, siStable siRNA half life is increased more than 500 fold over unmodified siRNA.

SiStable is available for in vitro cell culture applications. A version of the siRNA for in vivo animal applications is expected to be available in January, said Dharmacon.


The journal Oligonucleotides has released a special issue focusing entirely on RNA interference, according to the publishing company Mary Ann Liebert.

The issue includes articles on targeting CCR5 with siRNAs, strategies for generating siRNA libraries against the human genome, siRNA targeting Bcl-2, and tools for gene manipulation in chicken embryos, among others.

The issue is edited by John Rossi from the Beckman Research Institute of the City of Hope and Fritz Eckstein of the Max-Planck Institute for Experimental Medicine.

The issue is freely available at www.liebertpub.com/oli.


System Biosciences said this week that it has launched its GeneNet Lentiviral siRNA Transduction System based on the feline immunodeficiency virus.

According to the company, the delivery and expression vectors can permanently inhibit the expression of any gene in almost any mammalian cell or organism. The vectors, System Biosciences added, can be combined with cells expressing FIV proteins to create a pseudoviral particle that can carry siRNA coding sequences into mammalian cells.

Once inside the cell, a portion of the vector sequence will immediately integrate into the genome so that the cell permanently expresses the siRNA molecule, the company said.

The Scan

Study Finds Sorghum Genetic Loci Influencing Composition, Function of Human Gut Microbes

Focusing on microbes found in the human gut microbiome, researchers in Nature Communications identified 10 sorghum loci that appear to influence the microbial taxa or microbial metabolite features.

Treatment Costs May Not Coincide With R&D Investment, Study Suggests

Researchers in JAMA Network Open did not find an association between ultimate treatment costs and investments in a drug when they analyzed available data on 60 approved drugs.

Sleep-Related Variants Show Low Penetrance in Large Population Analysis

A limited number of variants had documented sleep effects in an investigation in PLOS Genetics of 10 genes with reported sleep ties in nearly 192,000 participants in four population studies.

Researchers Develop Polygenic Risk Scores for Dozens of Disease-Related Exposures

With genetic data from two large population cohorts and summary statistics from prior genome-wide association studies, researchers came up with 27 exposure polygenic risk scores in the American Journal of Human Genetics.