Ambion has recently introduced the MirVana Paris kit, which is designed to allow for the quantitative recovery of native protein and all RNA species, including miRNA and siRNA, from the same sample.
The kit, said Ambion, also includes a procedure to enrich the population of small RNAs to increase senstitivity in downstream analysis.
Details about the kit can be found at http://www.ambion.com/catalog/CatNum.php?1556.
Ambion also has introduced the Silencer siRNA Cocktail kit, which produces a cocktail of 21 to 23 base-pair siRNAs using Dicer.
The kit contains reagents to synthesize seven dsRNA cocktails, said Ambion, so that users can prepare five experimental siRNA cocktails, as well as both positive and negative control siRNA preps.
The kit includes reagents for transcription and cleanup of long dsRNA, Dicer enzyme and reaction buffer, and reagents for siRNA cleanup, Ambion said. Also included are templates for transcription of a positive control targeting GAPDH and a negative control siRNA.
Details about the kit can be found at http://www.ambion.com/catalog/CatNum.php?1612.